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E1031

Anti-ERGIC-53/p58 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ERGIC53, Anti-F5F8D, Anti-FMFD1, Anti-LMAN1, Anti-MCFD1, Anti-MR60, Anti-gp58

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200 μL
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$665.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD07370310
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB CL
Citations:
58

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

mouse, rat, human

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells, western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells

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Anti-ERGIC-53/p58 antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

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Anti-ERGIC-53/p58 antibody produced in rabbit

Anti-ERGIC-53 antibody, Mouse monoclonal clone ERGIC-3, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200585

Anti-ERGIC-53 antibody, Mouse monoclonal

Anti-Protein Disulfide Isomerase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

P7496

Anti-Protein Disulfide Isomerase antibody produced in rabbit

Anti-Cyclophilin B antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody

Sigma-Aldrich

SAB4200201

Anti-Cyclophilin B antibody produced in rabbit

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

UniProt accession no.

P49257

UniProt accession no.

P49257

UniProt accession no.

P07237

UniProt accession no.

P23284

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

ERGIC (ER-Golgi intermediate compartment) is related to type I membrane marker protein ERGIC-53 and rat homolog is referred as (p58) . ERGIC is a dynamic membrane system made up of tubulo vesicular clusters near to ER exit site which facilitates transport of protein from ER to Golgi. Anti-ERGIC-53/ (p58) antibody can be used as a primary antibody (diluted 1: 125) in fluorescence microscopy. It may also be used for western blotting. Rabbit anti-ERGIC-53/ (p58) antibodies react specifically with ERGIC-53/ (p58) of rat, mouse and human.

Immunogen

synthetic peptide corresponding to amino acids 158-170 of rat p58 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and human.

Application

Anti-ERGIC-53/ (p58) antibody can be used in immunoblotting and immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000476899
0000476899
0000433980
0000433980
0000188251

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Aaron B Bowen et al.
eLife, 6 (2017-09-07)
Neurons face the challenge of regulating the abundance, distribution and repertoire of integral membrane proteins within their immense, architecturally complex dendritic arbors. While the endoplasmic reticulum (ER) supports dendritic translation, most dendrites lack the Golgi apparatus (GA), an essential organelle
A Schweizer et al.
The Journal of cell biology, 107(5), 1643-1653 (1988-11-01)
Purified Golgi membranes of the human intestinal adenocarcinoma cell line Caco-2 were used as an antigen to produce a monoclonal antibody, G1/93, which specifically labels a tubulovesicular compartment near the cis side of the Golgi apparatus, including the first cis-cisterna
J Saraste et al.
The Journal of cell biology, 105(5), 2021-2029 (1987-11-01)
A 58-kD cis-Golgi protein has been identified by generating polyclonal antibodies against heavy (cis) Golgi subfractions. Total microsomes isolated from rat pancreatic homogenates were subfractionated to yield a rough microsomal fraction (B1) and three smooth membrane subfractions (B2-B4) enriched in
AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.
Mary Cabell Jonas, Mariana Pehar
Journal of Cell Science, 23, 3378-3388 (2010)
Adam K Walker et al.
PloS one, 8(11), e81170-e81170 (2013-12-07)
In amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration, TAR DNA binding protein 43 (TDP-43) accumulates in the cytoplasm of affected neurons and glia, where it associates with stress granules (SGs) and forms large inclusions. SGs form in response to
View All Related Papers

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Global Trade Item Number

SKUGTIN
E1031-200UL04061838104984

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