The complete formulation of this product is considered proprietary. However, the critical component information is available. Sodium pyruvate is added to this formulation. The glucose concentration is 22.57 mM or 4.06294 g/L.
51800C
Claycomb Medium
without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
HL-1 Medium
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 mL | Available to ship TODAYfromMILWAUKEE | $220.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
description
For research or for further manufacturing use.
Quality Level
sterility
sterile-filtered
form
liquid
storage condition
protect from light
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
Application
Claycomb Medium, named after Dr. William Claycomb who established the HL-1 cell line, is specifically designed for the growth of murine cardiomyocytes. HL-1 is the first cell line established that can maintain the differentiated cardiomyocyte phenotype and contractile activity in vitro. The HL-1 cell line can be used for the study of cardiac cell hypertrophy that follows myocardial infarction, the testing of novel cardiac therapeutic drugs and treatments, the production of high levels of cardiac proteins and the study of mature cardiomyocyte specific genes. Claycomb Medium, when supplemented with 100 μM norepinephrin, 10% fetal bovine serum (FBS) and 4 mM L-glutamine, will maintain the HL-1 cell line and the mature cardiomycyte behavior. While observing the HL-1 cells under light microscopy, individual and groups of cells can be observed contracting, becoming more frequent as the cardiomyocytes reach confluency.
1 of 1
This Item | |||
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| form liquid | form liquid | form liquid | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components L-glutamine: no | components glucose: 2.84 g/L | components L-glutamine: 292 mg/L | components Earle’s salts (5% CO2): yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Malou van den Boogaard et al.
Cell reports, 28(10), 2704-2714 (2019-09-05)
The human ether-a-go-go-related gene KCNH2 encodes the voltage-gated potassium channel underlying IKr, a current critical for the repolarization phase of the cardiac action potential. Mutations in KCNH2 that cause a reduction of the repolarizing current can result in cardiac arrhythmias
L A Sikkink et al.
Cell death & disease, 1, e98-e98 (2010-01-01)
Light-chain amyloidosis (AL) is a devastating protein-misfolding disease characterized by abnormal proliferation of plasma cells in the bone marrow that secrete monoclonal immunoglobulin light chains that misfold and form amyloid fibrils, thus causing organ failure and death. Numerous reports on
Jan Hendrik van Weerd et al.
eLife, 9 (2020-07-17)
Genome-wide association studies have implicated common genomic variants in the gene desert upstream of TBX3 in cardiac conduction velocity. Whether these noncoding variants affect expression of TBX3 or neighboring genes and how they affect cardiac conduction is not understood. Here
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 51800C-500ML | 04061832541808 |
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What type of Dulbecco’s modified Eagle’s medium is used as the basal medium here? Is it high glucose or low glucose? Does it have sodium pyruvate?
1 answer-
Helpful?
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