Deoxynucleoside Triphosphate Set

This product contains 4 separate vials of dATP, PCR Grade, dCTP, PCR Grade, dGTP, PCR Grade, and dTTP, PCR Grade, each with a concentration of 100 mM and as a clear, colorless solution of the sodium salt (pH 8.3).

The Deoxynucleoside Triphosphate Set, PCR Grade, is suitable for many applications where high-quality reagents are required. Such applications include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.[1][2]

PCR Grade Nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

• PCR Grade Nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
• They are provided as clear, colorless solutions in water at a concentration of 100 mM (pH 8.3).
• Due to the manufacturing process, PCR Grade Nucleotides are free of dNTPs with modified bases, as well as tetra- and pyrophosphate contamination. Tetraphosphates can disintegrate and form pyrophosphate, which is a known inhibitor of amplification reactions. Therefore, the purity of nucleotides is critical to the success of your amplification reactions.
• PCR Grade Nucleotides are stable for 30 months after the production date when stored at -15 to -25°C.

1 set containing 4 components

• dATP, PCR Grade, sodium salt, 100 mM
• dCTP, PCR Grade, sodium salt, 100 mM
• dGTP, PCR Grade, sodium salt, 100 mM
• dTTP, PCR Grade, sodium salt, 100 mM

Working solution: For best results, prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Function tested using RT-PCR: Tested for absence of contaminating deoxyribonucleases, ribonucleases, and nicking activities according to the current Quality Control procedures.

For life science research only. Not for use in diagnostic procedures.