If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
777323
Cy5-azide
95% (HPLC)
Synonym(s):
Cy5 Azide Fluorescent Probe
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mg | Available to ship TODAYfromMILWAUKEE | $180.00 | |
| 5 mg | Available to ship TODAYfromMILWAUKEE | $498.00 |
About This Item
Quality Level
assay
95% (HPLC)
form
solid
reaction suitability
reaction type: click chemistry
ε (extinction coefficient)
251,000
fluorescence
λex 647 nm; λem 663 nm
storage temp.
−20°C
SMILES string
CC1(C)C(/C=C/C=C/C=C(N2CCCS([O-])(=O)=O)\C(C)(C)C3=C2C=CC(S([O-])(=O)=O)=C3)=[N+](CCCCCC(NCCCN=[N+]=[N-])=O)C4=C1C=C(S([O-])(=O)=O)C=C4.CC[NH+](CC)CC.CC[NH+](CC)CC
InChI
1S/C37H48N6O10S3.2C6H15N/c1-36(2)29-25-27(55(48,49)50)16-18-31(29)42(22-10-6-9-15-35(44)39-20-11-21-40-41-38)33(36)13-7-5-8-14-34-37(3,4)30-26-28(56(51,52)53)17-19-32(30)43(34)23-12-24-54(45,46)47;2*1-4-7(5-2)6-3/h5,7-8,13-14,16-19,25-26H,6,9-12,15,20-24H2,1-4H3,(H3-,39,44,45,46,47,48,49,50,51,52,53);2*4-6H2,1-3H3
InChI key
NJSWGOLBDDXJQO-UHFFFAOYSA-N
Application
Solubility: DMSO, DMF, MeOH
1 of 1
This Item | |||
|---|---|---|---|
| assay 95% (HPLC) | assay 90% (HPLC) | assay ≥90% (HPLC) | assay 95% (HPLC) |
| reaction suitability reaction type: click chemistry | reaction suitability reaction type: click chemistry | reaction suitability reaction type: click chemistry | reaction suitability reaction type: click chemistry |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| form solid | form solid | form solid | form solid |
| fluorescence λex 647 nm; λem 663 nm | fluorescence - | fluorescence - | fluorescence - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 777323-1MG | 04061826688748 |
| 777323-5MG | 04061833231845 |
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Hi, I am trying to conjugate cy5 to a protein already has azide at its C-terminus. What would the protocol would be in this case? Do we need to use CuAAC in our case?
1 answer-
This product is a Cy5-azide. Conjugation may not be possible if the protein of interest has a terminal azide rather than a terminal alkyne. Should this be the case, a Cy5-alkyne such as product 777358, would be recommended. Click chemistry relies on 1,3-dipolar cycloaddition of alkynes to azides to form 1,4-disubsituted-1,2,3-triazoles.
Please see the link below to review an introduction to chemistry:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/chemistry-and-synthesis/adc-and-bioconjugation/click-chemistryPlease see the link below to review product 777358, Cy5-alkyne:
https://www.sigmaaldrich.com/product/aldrich/777358A search of the scientific literature may be carried out to develop the intended application. The suggested key terms of interest may be azide, alkyne, and click chemistry.
Helpful?
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