This application may be possible. However, it has not been specifically tested.
R0901
RNAlater®
Stabilize and protect RNA with immediate RNase inactivation
RNAlater®
Synonym(s):
RNA preservation solution, RNA protection solution, RNA protector, RNA stabilizer, RNA storage solution, RNA transportation solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mL | Available to ship TODAYfromMILWAUKEE | $198.00 | |
| 500 mL | Available to ship TODAYfromMILWAUKEE | $678.00 |
About This Item
$198.00
Available to ship TODAYDetails
Subscriptions are currently unavailable.
Ships Every 4 weeks
Quality Level
SMILES string
N([C@@H]([C@H](O)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CCCNC(=N)N)CC(=O)N)C)CC(C)C)C
InChI
1S/C37H67N15O13/c1-16(2)14-23(50-28(57)17(3)46-33(62)24(15-25(39)54)51-30(59)20(38)8-6-12-44-36(40)41)32(61)47-18(4)29(58)52-27(19(5)53)34(63)48-21(10-11-26(55)56)31(60)49-22(35(64)65)9-7-13-45-37(42)43/h16-24,27,53H,6-15,38H2,1-5H3,(H2,39,54)(H,46,62)(H,
InChI key
DUCQRBNNHJNRMP-KDDVYCELSA-N
General description
RNAlater is compatible with most RNA isolation methods, including TRI Reagent and GenElute Total RNA isolation and mammalian mRNA isolation kits.
RNAlater is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNAlater for storage without jeopardizing the quality or quantity of RNA. RNAlater eliminates the need to immediately process tissue specimens or to freeze samples in liquid nitrogen for later processing. RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.
RNAlater has been extensively tested on several tissues from vertebrate species, including brain, heart, kidney, spleen, liver, testis, skeletal muscle, fat, lung, and thymus. RNAlater is also effective for E. coli, Drosophila, tissue culture cells, white blood cells, and some plants.
RNAlater is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNAlater for storage without jeopardizing the quality or quantity of RNA. RNAlater eliminates the need to immediately process tissue specimens or to freeze samples in liquid nitrogen for later processing. RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.
RNAlater has been extensively tested on several tissues from vertebrate species, including brain, heart, kidney, spleen, liver, testis, skeletal muscle, fat, lung, and thymus. RNAlater is also effective for E. coli, Drosophila, tissue culture cells, white blood cells, and some plants.
RNAlater can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s isolation kits.
Application
RNAlater® has been used to synthesize antisense RNA probe. It can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s GenElute™ isolation kits.
Biochem/physiol Actions
RNAlater is easy to use. Simply cut tissue samples to be stored so they are less than 0.5 cm in at least one dimension and submerge in 5 volumes of RNAlater. Small organs, such as rat kidney, liver or spleen can be stored in whole in RNAlater. When ready to isolate the RNA, remove the tissue from RNAlater and process as though just harvested. For cell storage, resuspend pelleted cells in a small amount of PBS before adding 5-10 volumes of RNAlater. Before preparing RNA, pellet cells and discard supernatant.
Features and Benefits
- No compromise in RNA quality following mRNA or total RNA isolation
- Rapidly permeates tissues to stabilize and protect cellular RNA
- Aqueous non-toxic solution allows downstream tissue processing
- Stabilizes samples at room temperature
Other Notes
RNAlater® is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNAlater eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
RNAlater is a registered trademark of Thermo Fisher Scientific, Inc.
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| R0901-500ML | 04061838262165 |
| R0901-100ML | 04061838256300 |
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Hello, is it possible to submerge samples (1mm3) of myocardium to RNA later, and then the next week into liquid nitrogen? As -80°C freezers are not available? Will it affect RNA stability and its modifications?
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What does RNAlater do to tissue like bone? Does it affect the mineral phase or not at all?
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The effects of RNAlater on solid tissues, such as bone, has not been determined. However, this product has been used successfully on bone samples in several publications. Please see the links below to review these articles:
Maintaining mRNA Integrity during Decalcification of Mineralized Tissues
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591421/An Improved Method for Isolating High-Quality RNA From Rat Bone at Room Temperature Without the Need for Specialized Equipment
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027952/Improved method for isolating high-quality RNA from mouse bone with RNAlater at room temperature
https://www.sciencedirect.com/science/article/pii/S2352187219300178Helpful?
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Are there any references available on use of this product for measuring microbes in rock and soil samples?
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The scientific literature offers information on isolating RNA from soil, with specific mention of RNALater. Relevant studies can be found in the PubMed Central database, including the articles with PMC reference numbers PMC4036013 and PMC2935057.
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Can it preserve RNA in saliva?
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RNAlater has been utilized with saliva samples and published in the academic literature. Please see the link below for an example.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229791
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How long is the incubation time in the reagent before moving on to RNA extraction?
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The recommended incubation period is specified as overnight at 2-8°C.
Please see the product data-sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/789/r0901bul.pdfHelpful?
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Is there a problem if I store it in the fridge? at 4°C
1 answer-
RNAlater has been validated for storage in room temperature. When in use, the product is stored with samples refrigerated, as well as frozen. Storing the product at 4°C should not pose a problem. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/789/r0901bul.pdfHelpful?
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If protein is extracted from RNA later treated tissues are posttranslational modifications (e.g. phosphorylation) influenced by the reagent?
1 answer-
The impact of RNAlater on post-translational modifications has not been investigated, however the article below may be helpful.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397022/Helpful?
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Does RNAlater stop transcription upon addition or only preserves the RNA from degradation by RNAases?
1 answer-
RNAlater™ is used to stabilize and protect RNA with immediate RNase inactivation. It works by denaturing proteins, including RNase. The use of RNAlater™ RNA Stabilization Solution for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods. It is worth noting that proteins will be preserved in RNAlater™ however, the solution will denature the proteins. Therefore, the protein obtained from samples stored in it will still be suitable for applications such as western blotting or 2D gel electrophoresis, but not for applications that require native protein.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the shelf life of R0901, RNAlater®?
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R0901 does not have a specified retest or expiration date. We guarantee the product for 1 year from the date of receipt, according to our Product Dating and Shipping document.
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