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Sigma-Aldrich

4-Methylumbelliferyl oleate

lipase substrate, ≥95% (HPCE), solid, suitable for fluorescence

Synonym(s):

Oleic acid 4-methylumbelliferyl ester

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About This Item

Empirical Formula (Hill Notation):
C28H40O4
CAS Number:
Molecular Weight:
440.61
Beilstein/REAXYS Number:
4887028
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.21

product name

4-Methylumbelliferyl oleate, suitable for fluorescence, ≥95% (HPCE)

assay

≥95% (HPCE)

form

solid

storage condition

protect from light
under inert gas

mp

37-38 °C (lit.)

solubility

2-methoxyethanol: soluble
DMSO: soluble
chloroform: soluble

fluorescence

λex 312 nm in methanol
λex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase)

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C28H40O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(29)31-24-19-20-25-23(2)21-28(30)32-26(25)22-24/h10-11,19-22H,3-9,12-18H2,1-2H3/b11-10-

InChI key

NKQFKJYKCVDLPT-KHPPLWFESA-N

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Application

4-Methylumbelliferyl oleate has been used:
  • as a substrate to assay the activity of non-specific esterases
  • to determine lipase activity
  • as a substrate to evaluate pancreatic lipase inhibitory activity

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Fluorogenic substrate for lipase

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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T J Van Berkel et al.
Biochimica et biophysica acta, 617(3), 446-457 (1980-03-21)
(1) Parenchymal and non-parenchymal cells were isolated from rat liver. The characteristics of acid lipase activity with 4-methylumbelliferyl oleate as substrate and acid cholesteryl esterase activity with cholesteryl[1-14C]oleate as substrate were investigated. The substrates were incorporated in egg yolk lecithin
Non-flavonoid phenolics from Averrhoa carambola fresh fruit
Jia X, et al.
Journal of functional foods, 32(1), 419-425 (2017)
Thavaree Thilavech et al.
BMC complementary and alternative medicine, 19(1), 242-242 (2019-09-07)
Cyanidin-3-rutinoside (C3R), a naturally occurring anthocyanin, possesses anti-oxidant, anti-hyperglycemic, anti-glycation and cardioprotective properties. However, its mechanisms responsible for anti-hyperlipidemic activity have not been fully identified. The aim of the study was to investigate the lipid-lowering mechanisms of C3R through inhibition
Ola Abdelhedi et al.
Marine drugs, 17(4) (2019-04-03)
In this study, the antihypertensive activity of Purafect®-smooth hound viscera protein hydrolysate (VPH) and its peptide fraction with molecular weight (MW) below 1 kDa (VPH-I) was investigated. In addition, the lipase inhibitory activity, as well the anticoagulant potential, in vitro
Tao Chen et al.
Meat science, 84(1), 143-146 (2010-04-09)
In order to reveal the relationship between phospholipase A(2) and antioxidant enzymes and drip loss in pork, the study was designed to examine the effects of phospholipase A(2) and antioxidant enzymes on the water-holding capacity of pork during postmortem chilling.

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