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N2752

Sigma-Aldrich

4-Nitrophenyl palmitate

lipase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

p-Nitrophenyl palmitate, Hexadecanoic acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C22H35NO4
CAS Number:
1492-30-4
Molecular Weight:
377.52
Beilstein/REAXYS Number:
1891754
EC Number:
216-084-9
MDL number:
MFCD00047732
UNSPSC Code:
12352204
PubChem Substance ID:
24897590
NACRES:
NA.83

product name

4-Nitrophenyl palmitate, lipase substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

chloroform: 100 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CCCCCCCCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3

InChI key

LVZSQWIWCANHPF-UHFFFAOYSA-N

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General description

4-Nitrophenyl palmitate is a substrate for lipase enzyme activity. Lipase hydrolyzes 4-nitrophenyl palmitate and yields the yellow colored product 4-nitrophenol, which is measurable spectrophotometrically at 410 nm. This method is advantageous due to its short reaction time and facile spectrophotometric analyses. The cell-bound lipase has preference for 4-nitrophenyl palmitate as substrate than the extracellular lipase.

Application

4-nitrophenyl palmitate has been used as a substrate for lipase enzyme activity.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

pcodes

P280

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M M Maia et al.
Bioresource technology, 76(1), 23-27 (2001-04-24)
Lipase (Glycerol ester hydrolase EC 3.1.1.3.) from a Brazilian strain of Fusarium solani FSI has been investigated. The effect of different carbon sources and trace elements added to basal medium was observed with the aim of improving enzyme production. Lipase
Shamoon Asmat et al.
Materials science & engineering. C, Materials for biological applications, 99, 25-36 (2019-03-21)
Herein, as a promising support, a magnetic enzyme nanoformulation have been designed and fabricated by a poly-o-toluidine modification approach. Owing to the magnetic nature and the existence of amine functionalized groups, the as-synthesised poly(o-toluidine) functionalized magnetic nanocomposite (Fe3O4@POT) was employed
Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application
Zarinviarsagh M, et al.
Lipids in Health and Disease, 16(1), 177-177 (2017)
Seon-Woo Lee et al.
Applied microbiology and biotechnology, 65(6), 720-726 (2004-09-15)
The construction and screening of metagenomic libraries constitute a valuable resource for obtaining novel biocatalysts. In this work, we present the construction of a metagenomic library in Escherichia coli using fosmid and microbial DNA directly isolated from forest topsoil and
Differences in hydrolytic abilities of two crude lipases from Geotrichum candidum 4013
Brabcova J, et al.
Yeast, 27(12), 1029-1038 (2010)
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