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N9876

Sigma-Aldrich

4-Nitrophenyl butyrate

lipase substrate, chromogenic, esterase substrate, ≥98%, liquid

Synonym(s):

p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C10H11NO4
CAS Number:
2635-84-9
Molecular Weight:
209.20
Beilstein/REAXYS Number:
1879729
MDL number:
MFCD00047734
UNSPSC Code:
12352204
PubChem Substance ID:
24897915
NACRES:
NA.83

product name

4-Nitrophenyl butyrate, ≥98%

assay

≥98%

form

liquid

density

1.19 g/mL at 20 °C (lit.)

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3

InChI key

DVDUMIQZEUTAGK-UHFFFAOYSA-N

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General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

Application

4-nitrophenyl butyrate has been used:
  • as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
  • as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
  • as a substrate to determine the effect of buffers and solvents on human carboxylesterase

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Li-Chun Wang et al.
Journal of food and drug analysis, 26(3), 973-984 (2018-07-07)
Obesity is associated with higher risks of developing diabetes and cardiovascular disease. Green tea, rich in polyphenolic compounds such as epigallocatechin gallate (EGCG) and epigallocatechin (EGC), has been shown to display anti-obesity effects. Houttuynia cordata leaves have also been shown
Nathalia S Rios et al.
Biochimica et biophysica acta. Proteins and proteomics, 1867(9), 741-747 (2019-06-16)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h
Lipase and esterase: to what extent can this classification be applied accurately?
Lopes DB, et al.
Food Sci. Technol., 31(3), 603-613 (2011)
Sarah K Parker et al.
PloS one, 4(1), e4281-e4281 (2009-01-27)
The cell wall of M. tuberculosis is central to its success as a pathogen. Mycolic acids are key components of this cell wall. The genes involved in joining the alpha and mero mycolates are located in a cluster, beginning with
Differential response to chemically altered polyethylene by activated mature human monocyte-derived macrophages
Xing S, et al.
Biomaterials, 23(17), 3595-3602 (2002)
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