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1.05231

Sigma-Aldrich

Fuchsin acid (C.I. 42685)

for microscopy Certistain®

Synonym(s):

Fuchsin acid (C.I. 42685)

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About This Item

Empirical Formula (Hill Notation):
C20H17N3Na2O9S3
CAS Number:
3244-88-0
Molecular Weight:
585.54
UNSPSC Code:
41116130
EC Index Number:
221-816-5

Quality Level

400

form

solid

composition

Dye, ≥60% spectrophotometric assay

pH

3-4 (20 °C, 10 g/L in H2O)

mp

>130 °C (decomposition)

solubility

10 g/L

bulk density

920 kg/m3

εmax

780-1310 at 540-549 nm in hydrochloric acid at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,23H,21-22H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2

InChI key

WZRZTHMJPHPAMU-UHFFFAOYSA-L

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General description

Fuchsin acid (C.I. 42685) for microscopy Certistain®, is a triarylmethane dye used, among others, in trichrome connective tissue stainings according to Mallory and van Gieson. Mallory developed a staining procedure for visualization of collagenous connective tissue, which has been modified and improved over the years.It enables a good differentiation of individual tissue components, provided that the tissue has been fixed in Zenker′s solution. As Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Hydrochloric acid 0.0025 mol/l): 540 - 549 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; hydrochloric acid 0.0025 mol/l): 780 - 1310
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. What is the difference between acid fuchsin and carbol fuchsin

    1 answer

    1. Acid Fuchsin is made by the controlled sulfonation of Basic Fuchsin (Pararosaniline chloride) and is primarily the trisulfonated end product. This material was initially used in dyeing fabric, paper, soaps, and photographic film. Over the years biological applications have been developed and include connective tissue and collagen staining. It is also a component in blood and plant staining preparations.

      Carbol Fuchsin is prepared from pararosaniline and phenol, 90% and 10% respectively. This product is used primarily in staining acid-fast bacilli and Nissl bodies. It is also used in the staining of chromosomes and interphase chromatin in the Ziehl-Neelseb technique. A Lillie-modified Ziehl-Neeksen technique is also used for acid-fast staining.

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