415049

Aniline Blue diammonium salt

certified by the Biological Stain Commission

• Synonym(s): Ink Blue R; 4-[4-[[4-(4-aminooxysulfonylanilino)phenyl]-[4-(4-aminooxysulfonylphenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonic acid
• Empirical Formula (Hill Notation): C₃₇H₃₂N₅O₉S₃
• CAS Number: 66687-07-8
• Molecular Weight: 786.87
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder, crystals or granules
• composition: Dye content, 65%
• color: brown to very dark brown, and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown
• ε (extinction coefficient): 15000 at 595-605 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• InChI: 1S/C37H31N5O9S3/c38-50-53(46,47)35-21-15-32(16-22-35)41-29-9-3-26(4-10-29)37(25-1-7-28(8-2-25)40-31-13-19-34(20-14-31)52(43,44)45)27-5-11-30(12-6-27)42-33-17-23-36(24-18-33)54(48,49)51-39/h1-24,40-41H,38-39H2,(H,43,44,45)
• InChI key: CSYADFXDZYPANK-UHFFFAOYSA-N

Description

General description

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. It is a mixture of methyl blue (Cl 42780, Acid blue 93) and water blue (Cl 42755, Acid blue 22).

Application

Aniline blue (nominally as methyl blue or water blue) is a component of commonly used polychrome stains. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

Suitability

Certified for use in Mallory′s connective tissue stain and Gomori′s one-step trichrome.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves