B0904

Basic Fuchsin

certified by the Biological Stain Commission, powder

• Synonym(s): Basic Red 9, Fuchsin basic, Magenta O, Pararosaniline
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891537
• NACRES: NA.47

Properties

• product name: Basic Fuchsin, special for flagella, certified
• grade: certified by the Biological Stain Commission
• Quality Level: 200
• type: special for flagella, certified
• form: powder
• composition: pararosaniline acetate, 3 parts ; pararosaniline chloride, 1 part
• color: green to very dark green
• pH: 5.0-6.0 (1 g/L)
• mp: 250 °C
• solubility: H₂O: 1 mg/mL
• density: 1.220 g/cm³
• λmax: 544 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp

Description

General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:

• Acid-fast staining with carbol-fuchsine
• Periodic Acid-Schiff method
• Feulgen technique for DNA staining
• ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
• Leifson′s method for bacterial flagella staining.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

Suitability

Certified for use in Leifson′s flagella stain.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H350
• pcodes: P201 - P308 + P313
• Hazard Classifications: Carc. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P2 (EN 143) respirator cartridges