T2807

Thymidine Phosphorylase, recombinant from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

• Synonym(s): Thymidine:orthophosphate deoxy-D-ribosyltransferase
• CAS Number: 9030-23-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00132426
• EC Number: 2.4.2.4 (BRENDA, IUBMB)
• Recombinant: expressed in E. coli
• Concentration: ≥500 units/mL

Properties

• recombinant: expressed in E. coli
• Quality Segment: 300
• form: buffered aqueous solution
• concentration: ≥500 units/mL
• UniProt accession no.: P07650
• storage temp.: 2-8°C
• Gene Information: Escherichia coli K12 ... deoA(948901)

Description

General description

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation. [1]

Application

Thymidine phosphorylase has been used in a study to assess TAS-102 treatment in patients with advanced solid tumors. [2] Thymidine phosphorylase has also been used in a study to investigate antitumor effects of the FP3 protein on patient-derived tumor tissue xenograft models of primary colon carcinoma. [3]

Biochem/physiol Actions

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway.

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

Physical form

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

Preparation Note

Cloned from E. coli and produced in overexpressing E. coli

Other Notes

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)