SAB4200367

Anti-FLVCR1 antibody, Mouse monoclonal

clone FL-58, purified from hybridoma cell culture

• Synonym(s): Anti-AXPC1, Anti-FLVCR, Anti-Ffline leukemia virus subgroup C cellular receptor 1, Anti-MFSD7B, Anti-PCA, Anti-PCARP
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: FL-58, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: human, rat, monkey, mouse
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using A549 cells; western blot: 2.0-4.0 μg/mL using extracts of Raji cells
• isotype: IgG2a
• UniProt accession no.: Q9Y5Y0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FLVCR1(28982)

Description

General description

Feline leukemia virus subgroup C receptor 1 (FLVCR1) is a member of cell surface receptors that bind feline leukemia virus, subgroup C.

Monoclonal Anti-FLVCR1 (mouse IgG2a isotype) is derived from the hybridoma FL-58 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to the C-terminus of human FLVCR1 conjugated to KLH.

Specificity

Monoclonal Anti-FLVCR1 recognizes human, monkey, mouse and rat FLVCR1 (~72 kDa).

Immunogen

peptide corresponding to the C-terminus of human FLVCR1, conjugated to KLH. The corresponding sequence is identical in monkey and bovine FLVCR1.

Application

Anti-FLVCR1 antibody may be used for immunoblotting at a working concentration of 2.0-4.0 μg/ml using Raji cell extracts. The antibody is suitable for immunofluorescence at a recommended concentration of 2.5-5.0 μg/ml using A549 cells.

Monoclonal Anti-FLVCR1 antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Human feline leukemia virus subgroup C receptor 1 (FLVCR1) is required to transport cytoplasmic heme and maintain intracellular heme concentration, that protects the developing erythroid cells from heme toxicity. Disruption of FLVCR1 is implicated in fatal congenital anemia called Diamond-Blackfan anemia, degeneration of neurons in retinitis pigmentosa and posterior column ataxia via dysregulation of heme homeostasis). Knockdown of FLVCR is associated with defective erythropoiesis and disturbed systemic iron homeostasis in mice. Feline leukemia viruses (FeLVs) are pathogenic retroviruses of cats that disrupt the function of host receptor and causes fatal anemia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable