HPA005785

Anti-CD44 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): CD44 Antibody - Anti-CD44 antibody produced in rabbit, Cd44 Antibody, Anti-CD44 antigen precursor antibody produced in rabbit, Anti-CDw44 antibody produced in rabbit, Anti-Epican antibody produced in rabbit, Anti-Extracellular matrix receptor-III antibody produced in rabbit, Anti-GP90 lymphocyte homing/adhesion receptor antibody produced in rabbit, Anti-HUTCH-I antibody produced in rabbit, Anti-Heparan sulfate proteoglycan antibody produced in rabbit, Anti-Hermes antigen antibody produced in rabbit, Anti-Hyaluronate receptor antibody produced in rabbit, Anti-PGP-1 antibody produced in rabbit, Anti-Phagocytic glycoprotein I antibody produced in rabbit
• MDL number: MFCD00164629
• UNSPSC Code: 12352203
• Human Protein Atlas Number: HPA005785
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• product line: Prestige Antibodies^® Powered by Atlas Antibodies
• form: buffered aqueous glycerol solution
• species reactivity: human
• enhanced validation: orthogonal RNAseq; Learn more about Antibody Enhanced Validation
• technique(s): immunoblotting: 0.04-0.4 μg/mL; immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:1000-1:2500
• immunogen sequence: DDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPR
• UniProt accession no.: P16070
• application(s): research pathology
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CD44(960)

Description

General description

The CD44 (cell-surface glycoprotein) gene encodes a transmembrane glycoprotein and has 20 exons. It is mapped to human chromosome 11p13.

Immunogen

CD44 antigen precursor recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Anti-CD44 antibody produced in rabbit has been used:

• in immunohistochemistry
• in western blotting
• in immunofluorescence staining to characterize the immunophenotype of the mammospheres

Biochem/physiol Actions

CD44 is a hyaluronic acid receptor that regulates cell repair, adhesion and inflammation. This protein has been implicated in cancer aggressiveness, metastasis and growth. Thus, CD44 is used as a cell-surface marker for several cancers . Anti-CD44 antibody is specific for CD44 in humans.

Overexpression of CD44 (cell-surface glycoprotein) leads to esophageal squamous cell carcinoma (ESCC). It is involved in cell-cell and cell-extracellular matrix interactions. It plays an important role in lymphocyte homing and lymphocyte activation.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST83079

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)