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R3652

Sigma-Aldrich

Raf-1 Protein Kinase human

recombinant, expressed in E. coli, ~20 units/mg protein

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About This Item

MDL number:
MFCD04041081
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

form

solution

specific activity

~20 units/mg protein

mol wt

74 kDa

UniProt accession no.

P04049

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... RAF1(5894)

Application

Raf-1 is a serine/threonine protein kinase which is involved in the MAPK signal transduction pathway, a pathway that is involved in the development and progression of cancer. Raf-1 kinases are used to study potential cancer treatments as well as the responses to cancer treatments [1].

Biochem/physiol Actions

Raf-1 activates the protein kinases MEK1 and MEK2. MEK1 and MEK2 are phosphorylated to activate protein kinases ERK1 and ERK2. Activated ERKs play an important role in cell division, apoptosis, cell differentiation, and cell migration [2].

Unit Definition

One unit will activate MEK B substrate by 10% per min at pH 7.0 at 30 °C.

Physical form

Solution in 50 mM Tris-HCl, pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 10% glycerol.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBZ8348
SLBW3424
SLBQ2587V
031M1842
063K0328

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Raf kinase as a target for anticancer therapeutics
Sridhar SS, Hedley D, et al.
Clinical Cancer Research, 4, 677-685 (2005)
Liane M McGlynn et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 15(4), 1487-1495 (2009-02-21)
The expression and activation of the Ras/Raf-1/mitogen-activated protein kinase (MAPK) pathway plays an important role in the development and progression of cancer, and may influence response to treatments such as tamoxifen and chemotherapy. In this study we investigated whether the

Questions

1–3 of 3 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. Does Product R3652, Raf-1 Protein Kinase human, contain a GST tag?

    1 answer

    1. Yes. Product R3652, Raf-1 Protein Kinase human, contains a GST tag on the amino terminal end.

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