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I5278

Sigma-Aldrich

Insulin-Like Growth Factor Binding Protein-3 human

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, ≥95% (SDS-PAGE)

Synonym(s):

IGF Binding Protein-3, IGFBP-3

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About This Item

MDL number:
MFCD00285941
UNSPSC Code:
51111800
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in NSO cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

0.01-0.25 μg/mL

mol wt

29 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P17936

storage temp.

−20°C

Gene Information

human ... IGFBP3(3486)

Biochem/physiol Actions

Human IGFBP-3 is the major insulin-like growth factor (IGF) binding protein in plasma, where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).
IGFBPs (insulin like growth factor binding proteins) regulate bioavailability of IGFs (insulin like growth factors) in cells. Binding between IGFs and IGFBPs reduces the availability of the IGFs. However, IGFBPs also concentrate IGFs in specific areas to potentiate their effects at right time. IGFBP3 binds to IGF-1. It is a tumor suppressor and is downregulated in many cancers. In serum, IGFs, IGFBP3 and the acid labile subunit (ALS) exist together as a complex. This complex is important for increasing the half life of serum IGFs.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA.

Analysis Note

The biological acitivity is measured as the ability to inhibit the biological activity of 14 ng/ml recombinant human IGF-I on MCF-7 cells.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Chuan-Wei Yang et al.
Journal of the American Medical Directors Association, 16(5), 365-370 (2014-12-31)
Previous studies have demonstrated the polymorphisms of insulinlike growth factor-1 (IGF-1) and its binding protein-3 (IGFBP3) genes could affect the circulating IGF-1 level. Moreover, the serum IGF-1 level was correlated with muscle size. This study aimed to explore the effect
Min Sun Kim et al.
Molecular and cellular biochemistry, 403(1-2), 107-113 (2015-02-11)
Nuclear factor-kappaB (NF-κB) is a transcription factor that is activated in various neoplasms, including gastric cancer. Insulin-like growth factor binding protein-3 (IGFBP-3) is a potent tumor suppressor and is significantly suppressed in a variety of cancers. Although IGFBP-3 has been
Kelley, K.M., et al.
The International Journal of Biochemistry & Cell Biology, 28, 619-637 (1995)
Spagnoli, A. and Rosenfeld, R.G.
Curr. Op. Endocrinology and Diabetes, 4, 1-1 (1997)
Shoshana Yakar et al.
The Journal of clinical investigation, 110(6), 771-781 (2002-09-18)
IGF-1 is a growth-promoting polypeptide that is essential for normal growth and development. In serum, the majority of the IGFs exist in a 150-kDa complex including the IGF molecule, IGF binding protein 3 (IGFBP-3), and the acid labile subunit (ALS).
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