Boil to dissolve the agar and adjust pH to 7.8±0.2. Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.
The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.
Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.
Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
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