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MP0030

Sigma-Aldrich

LookOut® Mycoplasma Elimination Kit

Synonym(s):

Mycoplasma contamination treatment

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

usage

 kit sufficient for 5 mL mycoplasma elimination

Quality Level

200

packaging

pkg of 1 kit

storage condition

protect from light

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

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General description

The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.

Application

The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.

Features and Benefits

  • Minimal cytotoxic properties of the reagents
  • Highly effective
  • Retention of normal cell characteristics

Suitability

The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.

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Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

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Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)

Articles

Why Use Antibiotics in Cell Culture?

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

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Protocols

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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