L8777 is recommended for cell culture because it is a sterile product.
L9379
Lectin from Phytolacca americana (pokeweed)
lyophilized powder
Synonym(s):
PWM, Pokeweed mitogen
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5 MG
$184.30
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$335.00
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$882.00
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5 MG
$184.30
10 MG
$335.00
40 MG
$882.00
About This Item
$184.30
List Price$194.00Web-Only Promotion
Check Cart for Availability
Recommended Products
sterility
aseptically filled
Quality Level
form
lyophilized powder
potency
≤0.3 μg/mL mitogenic activity
storage temp.
2-8°C
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General description
Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.[1]
Application
Lectin from Phytolacca americana (pokeweed) has been used:
- as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation[2][3]
- as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes[4]
- as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures[5]
Biochem/physiol Actions
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.[1]
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Physical form
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Analysis Note
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Articles
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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Is the product Pokeweed L9379 suitable for cell cultures from B lymphocytes? Or is better to use the product L8777? We culture bone marrow samples from B-cell dyscrasias for cytogenetic studies.
1 answer-
Helpful?
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how to prepare Pokeweed to be used as mitogen for B-ALL cytogenetics blood culture
1 answer-
This product can be dissolved in PBS and used at a concentration of up to 3 µg/mL for a mitogenic effect on human peripheral blood mononuclear cells. Concentrations higher than 3 µg/mL may be toxic to these cells.
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