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L7647

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type VI, lyophilized powder

Synonym(s):

Con A

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About This Item

CAS Number:
11028-71-0
EC Number:
234-258-2
MDL number:
MFCD00071069
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Canavalia ensiformis

Quality Level

200

type

Type VI

form

lyophilized powder

potency

≤20 μg per mL agglutination activity

impurities

Carbohydrate, essentially free
 salt, essentially free

solubility

water: 1 % (w/v)

storage temp.

−20°C

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Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for developing zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been also used in the preparation of protein loaded microgels to prevent enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Preparation Note

Highly purified by specific affinity chromatography.

Analysis Note

Agglutination activity: Determined from serial dilutions of a 1 mg/mL solution (this activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

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