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L3001

Sigma-Aldrich

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg solid

Synonym(s):

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

wheat (germ)

type

Type I

form

lyophilized powder

specific activity

5-15 units/mg solid

UniProt accession no.

Q8L6B0

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Gene Information

wheat ... lipase(543006)

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General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:
  • to prepare enzyme solution for the biodegradability test
  • to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
  • to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Quality

Contains acid phosphatase.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Ondřej Dlouhý et al.
Cells, 10(9) (2021-09-29)
Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been
Preparation and characterization of poly (ethylene glycol)-crosslinked reacetylated chitosans
Dal P A, et al.
Carbohydrate Polymers, 42(2), 201-206 (2000)
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Ondřej Dlouhý et al.
Cells, 10(9) (2021-09-29)
In Part I, by using 31P-NMR spectroscopy, we have shown that isolated granum and stroma thylakoid membranes (TMs), in addition to the bilayer, display two isotropic phases and an inverted hexagonal (HII) phase; saturation transfer experiments and selective effects of
Lipase: An Industrial Enzyme Through Metagenomics
Advances in Food and Nutrition Research, 78, 71-94 (2018)
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