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62301

Sigma-Aldrich

Lipase from Aspergillus niger

powder (fine), ~200 U/g

Synonym(s):

Lipase AP6

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About This Item

CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

100

form

powder (fine)

specific activity

~200 U/g

mol wt

Mr ~45000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol acetic acid per minute at pH 7.4 and 40 °C (triacetin, Cat. No. 90240 as substrate) 50 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40 °C

Other Notes

Selective acylation and deacylation of furanose and pyranose derivatives; Enantioselective hydrolysis of 2-methyl 3-acetoxy esters; Resolution of the diols of bicycloheptane and bicyclooctane by enzymic hydrolysis; Glyceride synthesis

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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H. Akita et al.
Tetrahedron Letters, 27, 5241-5241 (1986)
W.J. Hennen et al.
The Journal of Organic Chemistry, 53, 4939-4939 (1988)
A Brandt-Kjelsen et al.
Journal of animal physiology and animal nutrition, 98(3), 547-558 (2013-09-17)
Inorganic selenium (Se) in the form of selenite is applied to livestock to avoid Se deficiency. Selenite is, however, an artificial Se source in diets of unsupplemented chickens. It is therefore hypothesized that organic Se sources, such as Se-enriched yeast
M.K. Tahoun et al.
Microbios. Lett., 28, 133-133 (1985)
Marco Filice et al.
Nature protocols, 7(10), 1783-1796 (2012-09-08)
This protocol describes the regioselective deprotection of single hydroxyls in peracetylated monosaccharides and disaccharides by enzymatic or chemoenzymatic strategies. The introduction of a one-pot enzymatic step by using immobilized biocatalysts obviates the requirement to carry out tedious workups and time-consuming
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