The amount of plasma that can be processed using a 100 mL pack of Histopaque®-1077 depends on the specific protocol and the volume of blood being processed. Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. The detailed protocol can be found here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/284/292/h8889pis.pdf
H8889
Histopaque®-1077 Hybri-Max™
liquid, sterile-filtered, BioReagent, suitable for hybridoma
Synonym(s):
Histopaque 1077
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Estimated to ship TODAYfromALLENTOWN | $82.80 |
500 mL | Estimated to ship TODAYfromMILWAUKEE | $313.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352201
sterility
sterile-filtered
Quality Segment
product line
BioReagent
form
liquid
technique(s)
cell culture | hybridoma: suitable, single cell analysis: suitable
impurities
endotoxin, tested
density
1.077 at 25 °C (relative density)(lit.)
storage temp.
2-8°C
General description
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3.[1] This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Application
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form solution |
| technique(s) cell culture | hybridoma: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility aseptically filled |
| density 1.077 at 25 °C (relative density)(lit.) | density 1.077 | density 1.083 | density 1.077 g/mL |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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How many plasma can be processed through on 100 mL pack. And what all items will come with pack? detailed product information is required.
1 answer-
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Is there a product in your inventory that is comparable to the Corning product 25-072-CV?
1 answer-
Upon comparison of the formulation and product description, the comparable product from MilliporeSigma is identified as product number H8889. Both products, 25-072 and H8889, have a density of 1.077 g/ml and share the same formulation components, including Diatrizoic acid at 96.219 and Polysucrose 400 at 61.363.
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What is the formulation for H8889 Histopaque-1077 Hybri-Max?
1 answer-
The formulation for H8889 Histopaque-1077 Hybri-Max consists of 5.7% polysucrose 400 and 9% sodium diatrizoate.
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