GE17-5154-01

HiTrap^® DEAE Fast Flow

Cytiva 17-5154-01, pack of 5 × 5 mL

• Synonym(s): DEAE HiTrap Column, Fast Flow DEAE Column
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: diethylaminoethyl
• description: Ion Exchanger Type (value)
• packaging: pack of 5 × 5 mL
• manufacturer/tradename: Cytiva 17-5154-01
• parameter: 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: 6% cross-linked agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 2-14
• working range: 2-12
• suitability: suitable for bioprocess medium

Description

General description

HiTrap^® DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose^™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose^™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose^™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose^™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.

Features and Benefits

• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
• The industry standard for ion exchange chromatography.
• High flow rates and good scale-up potential.
• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
• Predictable scale-up

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids