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DFF100

DEAE–Sepharose™

Fast Flow

Synonym(s):

Diethylaminoethyl–Sepharose™

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50 ML

$290.00

100 ML

$457.00

$290.00

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About This Item

CAS Number:
57407-08-6
UNSPSC Code:
47101511
NACRES:
NA.56
MDL number:
MFCD00146630

Key Documents

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form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

Quality Level

200

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Show Differences

1 of 4

This Item
A50120DCL6B100CCF100
DEAE–Sepharose™ Fast Flow

Sigma-Aldrich

DFF100

DEAE–Sepharose™

DEAE–Sephadex®

Sigma-Aldrich

A50120

DEAE–Sephadex®

DEAE–Sepharose™ CL-6B

Sigma-Aldrich

DCL6B100

DEAE–Sepharose™

CM Sepharose™ Fast Flow

Sigma-Aldrich

CCF100

CM Sepharose™

pore size

~4,000,000 Da exclusion limit

pore size

~200,000 Da exclusion limit

pore size

~4,000,000 Da exclusion limit

pore size

4,000,000 exclusion limit (av. mol. wt.)

matrix

6% cross-linked agarose

matrix

cross-linked dextran

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

technique(s)

affinity chromatography: suitable

technique(s)

protein purification: suitable

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

capacity

110-160 μeq/mL binding capacity(gel volume)

capacity

3-4 meq/g binding capacity

capacity

130-170 μeq/mL binding capacity (gel volume)(gel volume)

capacity

90-130 μeq/mL(gel basis)

form

suspension

form

powder

form

suspension

form

suspension

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.
DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity[1]
  • to purify isoinhibitors
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

comparable product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000517672
0000517701
0000517672
0000517701
MKCZ8075

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Growth cone collapse and neurite retraction from cultured <I>Helisoma</I> neurons in response to antibody Fab fragments against an extracellular matrix protein.
Miller, J.D., et al.
Dev. Brain Res., 79(2), 203-218 (1994)
Valérie Molinier-Frenkel et al.
Journal of virology, 76(1), 127-135 (2001-12-12)
The capacity of recombinant adenoviruses (rAd) to induce immunization against their transgene products has been well documented. In the present study, we evaluated the vaccinal adjuvant role of rAd independently of its vector function. BALB/c mice received one subcutaneous injection
Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems
Rosenberg Y, et al.
Testing, 8(3), e58724-e58724 (2013)
Effective Purification Procedure of <I>Aspergillus oryzae</I> α−Amylase from Solid State Fermentation Cultures Including Concanavalin-A Sepharose
Terebiznik, M.R., et al.
Journal of Food Biochemistry, 19(5), 341-354 (1995)
G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
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