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Key Documents

G8511

Supelco

β-Galactosidase from Escherichia coli

For use as a marker in SDS-PAGE

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.25

mol wt

~116 kDa

packaging

vial of 0.5 mg

storage temp.

2-8°C

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Application

β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).[1] It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.[2]

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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