G6282
Monoclonal Anti-β-Galactosidase antibody produced in mouse
clone GAL-40, ascites fluid
Synonym(s):
Mouse Anti-beta-Galactosidase
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GAL-40, monoclonal
contains
15 mM sodium azide
species reactivity
bacteria
technique(s)
dot blot: suitable using soluble β-galactosidase
immunocytochemistry: suitable
western blot: 1:1,000 using denatured-reduced Escherichia coli β-galactosidase
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
β-Galactosidase is coded by the LacZ gene which helps to transform lactose into galactose.
β-galactosidase is a ~465 kDa tetrameric protein. Eukaryotic genes are usually cloned into E.coli β-galactosidase (lacZ) gene that results in a fusion hybrid of the desired gene product with the β-galactosidase enzyme . As the amount of the desired gene product in these cloned vector systems corresponds to the amount of β-galactosidase, enzyme assays for β-galactosidase can be utilized to detect the required gene product . Monoclonal anti-β-galactosidase antibodies are specific for β-galactosidase in E.coli and enable the analysis of β-galactosidase fusion proteins encoded by cloned vector systems.
Specificity
The antibody may be used for detection of β-galactosidase expressed by the E. coli lacZ gene encoded in many cloned gene sequences; serves as an indicator for fusion proteins encoded by an inserted DNA sequence.
Immunogen
β-D-galactosidase from E. coli
Application
Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used for western blotting.
Monoclonal anti-β-galactosidase antibodies may be used in dot blot (using soluble β-galactosidase), western blot (at a dilution of 1:1,000, using denatured-reduced Escherichia coli β-galactosidase) and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the stepwise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.
Biochem/physiol Actions
β-galactosidase catalyses the hydrolysis of lactose to form glucose and galactose. It serves as a reporter enzyme in a number of analysis such as studying the regulation of gene expression, protein function and structure determination, and examining of target gene expression. β-galactosidase is also known to yield coloured products by acting on several substrates. Thus, it is prefered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure, and target gene expression.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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K Hayashibe et al.
Journal of immunoassay, 11(1), 89-95 (1990-01-01)
A rapid and sensitive enzyme-linked immunoassay (ELISA) to quantitate recombinant fusion proteins encoded by cloned cDNA in the bacteriophage lambda gt11 is described. Since the fusion protein is expressed in an equimolar ratio to beta-galactosidase, the assay derives the concentration
P Dráber et al.
Hybridoma, 11(3), 385-390 (1992-06-01)
A panel of seven mouse monoclonal antibodies (BG-01-BG-07) was prepared against beta-galactosidase derived from E. coli. The antibodies are beta-galactosidase specific, show no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beta-galactosidase fusion
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