We were trying to use this as a protein standard and found it to be a heterogeneous mess. Not sure what all is in here, but its a lot more than betagalactosidase
G6008
β-Galactosidase from Escherichia coli
β-Galactosidase from Escherichia coli
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Grade VI, lyophilized powder, ≥250 units/mg protein
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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1000 UNITS
$103.00
5000 UNITS
$303.00
10000 UNITS
$546.00
$103.00
Available to ship onApril 08, 2025Details
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1000 UNITS
$103.00
5000 UNITS
$303.00
10000 UNITS
$546.00
About This Item
$103.00
Available to ship onApril 08, 2025Details
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biological source
Escherichia coli
Quality Level
type
Grade VI
form
lyophilized powder
specific activity
≥250 units/mg protein
mol wt
465 kDa
does not contain
BSA as extender
composition
Protein, ≥50% biuret
shipped in
wet ice
storage temp.
−20°C
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General description
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.[1]
Application
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop[4].
Physical properties
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Unit Definition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Physical form
Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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