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C8003

5-α-Cholestane

≥97.0% (HPLC)

Synonym(s):

NSC 224419

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Pack SizeSKUAvailabilityPrice
100 mg

Estimated to ship onJune 02, 2026fromMILWAUKEE

$56.20
1 g

Available to ship TODAYfromMILWAUKEE

$229.00
5 g

Available to ship TODAYfromMILWAUKEE

$904.00

About This Item

Empirical Formula (Hill Notation):
C27H48
CAS Number:
Molecular Weight:
372.67
UNSPSC Code:
12352211
NACRES:
NA.77
PubChem Substance ID:
EC Number:
207-562-8
Beilstein/REAXYS Number:
2051806
MDL number:

$56.20


Estimated to ship onJune 02, 2026Details


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Quality Segment

assay

≥97.0% (HPLC)

form

powder

mp

80-82 °C (lit.)

SMILES string

CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC[C@H]4CCCC[C@]4(C)[C@H]3CC[C@]12C

InChI

1S/C27H48/c1-19(2)9-8-10-20(3)23-14-15-24-22-13-12-21-11-6-7-17-26(21,4)25(22)16-18-27(23,24)5/h19-25H,6-18H2,1-5H3/t20-,21-,22+,23-,24+,25+,26+,27-/m1/s1

InChI key

XIIAYQZJNBULGD-XWLABEFZSA-N

Application

5-α-Cholestane has been used:
  • as an internal standard in gas phase chromatography (GC) and high-performance liquid chromatography (HPLC) to quantify cholesterol in meat products for a comparison study
  • to quantify the active compounds in the oils using a gas chromatograph coupled to a mass spectrometer
  • as a reference standard in GC analyses to quantify sterol content in the unsaponifiables obtained from oat lipids and different sterol standards like fucosterol, sitosterol, campesterol, and stigmasterol

Biochem/physiol Actions

5α-Cholestane is a sterol produced endogenously from cholesterol and has been isolated from human feces. It is derived from cholesterol by the action of intestinal microorganisms.[1] Derivatives of 5α-cholestane in plants are called brassinosteroids that selectively activate the PPI3K/Akt pathway.[2]

Preparation Note

5α-Cholestane yields clear to slightly hazy, colorless to faint yellow solution in chloroform at 50 mg/ml.

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This Item
C96232099027370
assay

≥97.0% (HPLC)

assay

≥98% (HPLC)

assay

≥98.0%

assay

≥98.0% (NT)

form

powder

form

powder

form

-

form

powder

mp

80-82 °C (lit.)

mp

-

mp

682 °C (lit.)

mp

260-263 °C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Global Trade Item Number

SKUGTIN
C8003-1G04061838445667
C8003-5G04061838445674
C8003-100MG04061838445650

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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