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B3804

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BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonym(s):

5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Quality Level

100

form

liquid

storage temp.

2-8°C

Related Categories

General description

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.

Caution

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Other Notes

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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