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AEC101

Sigma-Aldrich

AEC Staining Kit

liquid

Synonym(s):

ACE substrate kit

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1 KIT
$154.80

$154.80

List Price$172.00Save 10%
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Available to ship onMay 01, 2025Details

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1 KIT
$154.80

About This Item

UNSPSC Code:
41116134
NACRES:
NA.32

$154.80

List Price$172.00Save 10%
Web-Only Promotion

Available to ship onMay 01, 2025Details

form

liquid

usage

sufficient for ≥1,000 tests (150 ml substrate solution)

storage temp.

2-8°C

Application

AEC Staining Kit has been used as a substrate
  • for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods[1]
  • for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay[2]
  • for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads[3]

Packaging

Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.

Caution

Stable at least one year at 2-8 °C.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

134.6 °F - closed cup

flash_point_c

57 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
0000410404
0000410404
0000328703
0000328703
0000210570

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Alessandra Petrelli et al.
The Journal of clinical investigation, 128(10), 4669-4681 (2018-09-11)
Chronic inflammatory diseases are characterized by recurrent inflammatory attacks in the tissues mediated by autoreactive T cells. Identity and functional programming of CD8+ T cells at the target site of inflammation still remain elusive. One key question is whether, in
Kaiyuan Ni et al.
Science advances, 6(40) (2020-10-04)
Cancer vaccines have been actively pursued to bolster antitumor immunity. Here, we designed nanoscale metal-organic frameworks (nMOFs) as locally activable immunotherapeutics to release danger-associated molecular patterns (DAMPs) and tumor antigens and deliver pathogen-associated molecular patterns (PAMPs) for in situ personalized
Kirti Kajal et al.
Scientific reports, 9(1), 4073-4073 (2019-03-13)
Vasculogenesis and angiogenesis are process of formation of blood vessels. Blood vessels are evolved to distribute nutrients and oxygen to distant organs. These vessels are crucial for growth and repair of wounded tissue. During tumor condition there occurs imbalance in
Asma Mohammad Karami et al.
Diseases of aquatic organisms, 128(3), 187-201 (2018-06-05)
Caspian trout Salmo trutta caspius is an endangered subspecies of brown trout Salmo trutta which is native to the Caspian Sea. Restocking programmes have been established, but recent introduction of the rhabdovirus viral haemorrhagic septicaemia virus (VHSV) into Iranian rainbow
Tianyi Tang et al.
The Journal of biological chemistry, 285(7), 4637-4644 (2009-12-19)
To study the metabolic activity of NF-kappaB, we investigated phenotypes of two different mouse models with elevated NF-kappaB activities. The transcriptional activity of NF-kappaB is enhanced either by overexpression of NF-kappaB p65 (RelA) in aP2-p65 mice or inactivation of NF-kappaB
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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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