This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.
D0426
DAB with Metal Enhancer
SIGMAFAST™, peroxidase substrate, tablet
Synonym(s):
3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate
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5 SETS
$52.70
50 SETS
$231.00
$52.70
Estimated to ship onApril 05, 2025
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5 SETS
$52.70
50 SETS
$231.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
$52.70
Estimated to ship onApril 05, 2025
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Product Name
SIGMAFAST™ DAB with Metal Enhancer, tablet
form
tablet
Quality Level
solubility
water: 1 tablet/5 mL
storage temp.
−20°C
General description
SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Application
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry,[1] dorsal root ganglion (DRG) neurons[2] and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.[3]
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
Reconstitution
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocols
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
1 answer-
Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
Helpful?
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What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
1 answer-
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
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