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A5588

Sigma-Aldrich

Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-polyHistidine, Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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0.5 ML
$875.50

$875.50

List Price$1,030.00Save 15%
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Available to ship onMay 02, 2025Details

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0.5 ML
$875.50

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

$875.50

List Price$1,030.00Save 15%
Web-Only Promotion

Available to ship onMay 02, 2025Details

Notify Me

Get notified when this item is ready to ship via email.

biological source

mouse

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

buffered aqueous glycerol solution

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

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General description

The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody is suitable for ELISA, immunoblotting and dot blotting assays.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000430829
0000430829
0000389409
0000389409
0000388870

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Jarrod J Mousa et al.
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A short disordered N-terminal flagellin segment is a fully functional flagellar type III export signal. Application for secreted protein expression
Dobo,J et al.
Applied and Environmental Microbiology (2009)
Sarah E Partovi et al.
The Journal of biological chemistry, 293(14), 5236-5246 (2018-02-08)
For nearly 30 years, coenzyme M (CoM) was assumed to be present solely in methanogenic archaea. In the late 1990s, CoM was reported to play a role in bacterial propene metabolism, but no biosynthetic pathway for CoM has yet been
Pooja Bhardwaj et al.
Antimicrobial agents and chemotherapy, 60(4), 2209-2221 (2016-01-27)
Chlorhexidine is a bisbiguanide antiseptic used for infection control. Vancomycin-resistantE. faecium(VREfm) is among the leading causes of hospital-acquired infections. VREfm may be exposed to chlorhexidine at supra- and subinhibitory concentrations as a result of chlorhexidine bathing and chlorhexidine-impregnated central venous
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