The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.
Specificity
The antibody detects transfected proteins contanining 6×His tag.
Immunogen
Synthetic peptide: 18XHis conjugated to KLH
Application
Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
The Anti-6-His antibody has been used in western blotting.
Biochem/physiol Actions
The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency
Wang T, et al.
Nature Cell Biology, 19(7), 833-833 (2017)
A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer.
Chen X, et al.
Theranostics, 8(9), 2348-2348 (2018)
Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation
Jiang Y, et al.
Nature Cell Biology, 17(9), 1158-1158 (2015)
The FLAG? peptide, a versatile fusion tag for the purification of recombinant proteins.
Einhauer A and Jungbauer A
Journal of Biochemical and Biophysical Methods, 49(1-3), 455-465 (2001)
Lourena E Costa et al.
International journal of molecular sciences, 20(8) (2019-04-25)
Two Leishmania infantum mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present
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