It is suggested to prepare the staining solution with a 1:10,000 dilution of this product in one of the following buffers:
• 1x TE buffer (10 mM Tris-HCl with 1 mM EDTA, pH 8.0)
• 1x TBE buffer (89 mM Tris base, 89 mM boric acid, and 1 mM EDTA, pH 8.0)
• 1x TAE buffer (40 mM Tris-acetate with 1 mM EDTA, pH 8.0)
Note: The buffer should not have been used previously for electrophoresis for optimal results. For optimal sensitivity, verify that the pH is between 7.5 and 8.0 (preferably pH 8.0) as SYBR Green I is pH sensitive.
S9430
SYBR™ Green I nucleic acid gel stain
10,000 × in DMSO
Synonym(s):
DNA stain, safer gel stain, SYBR™ green gel dye
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.5 mL | Estimated to ship TODAYfromSAINT LOUIS | $526.00 | |
| 1 mL | Available to ship TODAYfromMILWAUKEE | $957.00 |
About This Item
$526.00
Estimated to ship TODAYDetails
form
solution
Quality Segment
usage
mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
General description
SYBR™ Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.
Application
SYBR™ Green I nucleic acid gel stain has been used for:
- the quantification of dsDNA
- to stain DNA in polymerase chain reaction (PCR)
- for comet assay technique
- to assess spermatozoon membrane integrity
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)[1]
- as a fluorescent dye in flow cytometry[2]
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA[2]
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR™ Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Preparation Note
Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.
Legal Information
SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries
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This Item | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) - | technique(s) PCR: suitable | technique(s) PCR: suitable |
| concentration 10,000 × in DMSO | concentration 10,000 × in DMSO | concentration - | concentration - |
| form solution | form - | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level - |
| greener alternative category | greener alternative category | greener alternative category - | greener alternative category - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
201.2 °F - closed cup
flash_point_c
94 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| S9430-.5ML | 04061837899140 |
| S9430-1ML | 04061837899164 |
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Which final concentration is recommended for staining PCR products in agarose gel with UV transiluminator from de 10000x S9430 stock?
1 answer-
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Does Millipore Sigma offer SAFE Red loading dye?
1 answer-
SAFE Red loading dye is not offered by Millipore Sigma.
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What is the molarity of this product?
1 answer-
This product is a 10,000X concentrated solution suitable to prepare 10L of staining solution for in-gel staining of DNA and RNA. The specific concentration is considered proprietary. Published working concentrations for solutions of Sybr Green I range from 0.5 - 1.0 ug/ml. The molecular weight as published by Chebi, is 509.74 g/mol. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/282/803/s9430pis-ms.pdfPlease see the link below to review the Chebi profile:
https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:51461
chebi file
https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:51461 MW 509.74
Science direct
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/sybr-green-i
'working' concentration 0.5 - 1.0 ug/mlHelpful?
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Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?
1 answer-
Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine. A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine. This will allow for better staining.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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