33731

Trichloroacetic acid

puriss. p.a., ACS reagent, ≥99.5%

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• Beilstein/REAXYS Number: 970119
• EC Number: 200-927-2
• MDL number: MFCD00004177
• UNSPSC Code: 12352100
• PubChem Substance ID: 57648907

Properties

• grade: ACS reagent; puriss. p.a.
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• assay: ≥99.5%
• impurities: ≤0.002% heavy metals (as Pb); ≤0.005% water insolubles
• ign. residue: ≤0.01% (as SO₄)
• refractive index: n20/D 1.62 (lit.)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• cation traces: Cu: ≤5 mg/kg; Fe: ≤10 mg/kg
• suitability: complies for appearance of solution
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N

Description

General description

Trichloroacetic acid (TCA) is a chlorinated acetic acid. It is one of the major metabolite of trichloroethylene (TCE).[1]

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid may be used for the quantification of inorganic phosphorus in various biological samples (serum, urine and stool ash solutions).[2] It may be used for the elimination of acid-soluble and lipid phosphorous during the quantitative estimation of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues.[3] 10% trichloroacetic acid may be employed for the preparation of radioactive proteins.[4]

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H314,H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges