91230

Trichloroacetic acid

ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• Beilstein/REAXYS Number: 970119
• EC Number: 200-927-2
• MDL number: MFCD00004177
• UNSPSC Code: 12352100
• PubChem Substance ID: 57653429
• NACRES: NA.21

Properties

• grade: ACS reagent
• Quality Level: 200
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• assay: ≥99.5% (T); ≥99.5%
• form: flakes
• quality: for the determination of Fe in blood according to Heilmeyer
• ign. residue: ≤0.03% (as SO₄)
• refractive index: n20/D 1.62 (lit.)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubility: water: soluble 81.7 g/L at 20 °C
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤200 mg/kg
• cation traces: Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N

Description

General description

Trichloroacetic acid (TCA) is a chlorinated acetic acid. It is one of the major metabolites of trichloroethylene (TCE).

Application

Trichloroacetic acid may be used in the following studies:

• Estimation of phosphorus in various biological samples (serum, urine and stool ash solutions).
• Elimination of acid-soluble and lipid phosphorous during the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues.
• Preparation of 2-methyl-4-nitroanilinium trichloroacetate trichloroacetic acid (2M4NATCA) crystals.

10% Trichloroacetic acid may be employed for the preparation of radioactive proteins.

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Other Notes

Protein denaturant

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H314,H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges