MABS1180

Anti-E3 ubiquitin-protein ligase UBR1 Antibody, clone 6H9.1

clone 6H9.1, from mouse

• Synonym(s): EC: 2.3.2.27, N-recognin-1, RING-type E3 ubiquitin transferase UBR1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6H9.1, monoclonal
• species reactivity: human, rat
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_777576
• UniProt accession no.: Q8IWV7
• target post-translational modification: unmodified
• Gene Information: human ... UBR1(197131) ; rat ... Ubr1(499877)

Description

General description

E3 ubiquitin-protein ligase UBR1 (UniProt: Q8IWV7; also known as EC: 2.3.2.27, N-Recognin-1, RING-type E3 ubiquitin transferase UBR1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I) is encoded by the UBR1 gene (Gene ID: 197131) in human. E3 ubiquitin-protein ligase UBR1 is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It may also be involved in pancreatic homeostasis. Two isoforms of UBR1 have been described that are produced by alternative splicing. UBR1 is broadly expressed protein with highest levels reported in skeletal muscle, kidneys, and pancreas. UBR1 has two zinc finger domains. The UBR-type domain is localized to amino acids 97 to 168 and it forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for arginine in first position and displays poor affinity for histidine. The second zinc finger domain, RING-type, atypical, is localized to amino acids 1098-1201. Mutations in UBR1 gene are causative factor in Johanson-Blizzard syndrome that is characterized by congenital exocrine pancreatic insufficiency, malformations such as nasal wing aplasia, and mental retardation.

Specificity

Clone 6H9.1 detects E3 ubiquitin-protein ligase UBR1 in human and rat cells. It targets an epitope within 101 amino acids from the internal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human E3 ubiquitin-protein ligase UBR1.

Application

Anti-E3 ubiquitin-protein ligase UBR1, clone 6H9.1, Cat. No. MABS1180, is a mouse monoclonal that detects E3 ubiquitin-protein ligase UBR1 and has been tested for use in Immunohistochemistry (Paraffin) Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected E3 ubiquitin-protein ligase UBR1 in human pancreas and human kidney tissue sections.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected E3 ubiquitin-protein ligase UBR1 in C6 cell lysate.

Research Category
Signaling

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UBR1 in HEK293 cell lysate.

Target description

~200 kDa observed; 200.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable