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D218200

5,5′-Dithiobis(2-nitrobenzoic acid)

ReagentPlus®, 99%

Synonym(s):

3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

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Pack SizeSKUAvailabilityPrice
1 g
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$36.20
5 g
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$86.90
10 g
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$156.00

About This Item

Linear Formula:
[-SC6H3(NO2)CO2H]2
CAS Number:
Molecular Weight:
396.35
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
200-714-4
Beilstein/REAXYS Number:
1896821
MDL number:
Assay:
99%
Form:
powder

$36.20


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Quality Segment

product line

ReagentPlus®

assay

99%

form

powder

mp

240-245 °C (dec.) (lit.)

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

General description

DTNB (Ellman’s reagent) is commonly used for quantification of sulfhydryl (thiol) groups in proteins and biomolecules.

Application

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
D8130N21022373605
assay

99%

assay

≥98%

assay

-

assay

85%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

mp

240-245 °C (dec.) (lit.)

mp

240-245 °C (dec.) (lit.)

mp

-

mp

263-265 °C (lit.)

form

powder

form

powder

form

powder

form

solid

product line

ReagentPlus®

product line

BioReagent

product line

-

product line

-


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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

wgk

WGK 2



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Pork proteins oxidative modifications under the influence of varied time-temperature thermal treatments: A chemical and redox proteomics assessment.
Mitra B, et al.
Meat Science, 140, 134-144 (2018)
Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid
W Chen, et al.
Journal of Pharmaceutical and Biomedical Analysis, 48, 1375-1380 (2008)
Atef M K Nassar et al.
Molecules (Basel, Switzerland), 25(11) (2020-06-11)
Sumithion (Fenitrothion) (SUM) is an organophosphorus insecticide used to combat a wide variety of plant pests. Exposure to SUM causes significant toxicity to the brain, liver, kidney, and reproductive organs through, for example, binding to DNA, and it induces DNA



Global Trade Item Number

SKUGTIN
D218200-5G04061835282203
D218200-1G04061835282197
D218200-10G04061835282180

Questions

1–3 of 3 Questions  
  1. 1) What is the ideal storage temperature for long periods of stability? 2) How long does the product remain stable after manufacturing?

    1 answer
    1. This product can be stored at room temperature. Neither a recommended retest date nor an expiration date is assigned to this product, which means that it would fall under the general warranty of one year from date of shipment.

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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