T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

• Numéro CAS: 52500-60-4
• Numéro MDL: MFCD00132423
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: Escherichia coli
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: ≥90% (SDS-PAGE)
• Forme: essentially salt-free, lyophilized powder
• Activité spécifique: ≥3 units/mg protein
• Poids mol.: 11.7 kDa
• Technique(s): activity assay: suitable
• Solubilité: water: 1.90-2.10 mg/mL, clear, colorless
• Numéro d'accès Protein ID: M26133
• Numéro d'accès UniProt: P0AA25
• Application(s): cell analysis
• Température de stockage: −20°C
• Informations sur le gène: Escherichia coli K12 ... trxA(948289)

Description

Description générale

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Actions biochimiques/physiologiques

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

Autres remarques

GenBank^® Accession Number M26133.

Définition de l'unité

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Informations légales

GenBank is a registered trademark of United States Department of Health and Human Services

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)