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CS0170

Sigma-Aldrich

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Synonyme(s) :

Thioredoxin Reductase Activity Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

200

Utilisation

 kit sufficient for 100 assays (1 mL)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)

Catégories apparentées

Description générale

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Caractéristiques et avantages

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Composants de kit seuls

Réf. du produit
Description
  • Assay Buffer 5x for Thioredoxin Reductase 30 mL
  • Thioredoxin Reductase 10 μg
  • Thioredoxin Reductase Inhibitor Solution .05 mL
  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
  • NADPH 25 mg
  • Dimethyl Sulfoxide (DMSO) 7.5 mL

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H361

Classification des risques

Repr. 2

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C

Certificats d'analyse (COA)

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