PPB001

Solution saline tamponnée au Tris

powder, pH 8.0, pHast Pack^™

• Synonyme(s) : TBS
• Numéro MDL: MFCD00132476
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.21

Propriétés

• product name: Solution saline tamponnée au Tris, pH 8.0, pHast Pack^™, powder
• Gamme de produits: pHast Pack^™
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: ≤5 ppm Heavy Metals (lead)
• Couleur: white
• pH: 8.0
• Solubilité: soluble, clear, colorless
• Traces de cations: Fe: <10 ppm
• Activité étrangère: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• Température de stockage: room temp

Description

Description générale

Tris Buffered Saline, pH 8.0 is a commonly used wash buffer that is isotonic and nontoxic and suitable for use in cell culture and electrophoresis applications.

Application

Tris-buffered saline is intended to use for:

• Sample preparation
• ELISA
• Western Blot and other protein applications
• Cell culture (recommend to sterile filter)
• Immunohistochemistry
• Resuspension and sonication of cells

Caractéristiques et avantages

• Ready-to-use buffer – fast and easy to prep
• No need to measure or adjust pH, simply add water
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Conditionnement

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 50mM Tris, 138mM NaCl, 2.7mM KCl, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations légales

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable