PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• Synonyme(s) : 0.1M Tris
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Gamme de produits: pHast Pack^™
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: ≤5 ppm Heavy Metals (lead)
• Couleur: white
• pH: 8.1
• Traces de cations: Fe: ≤10 ppm
• Activité étrangère: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Température de stockage: room temp

Description

Description générale

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Application

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

Caractéristiques et avantages

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Conditionnement

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

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Informations légales

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1