Principaux documents

Voir toute la documentation

Passer à

PPB006

Solution tampon phosphate

Name: Solution tampon phosphate
Brand: SIAL

powder, pH 7.4, pHast Pack^™

Synonyme(s) :

PBS

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue

À vous/Référence	Disponibilité	Prix

A propos de cet article

UNSPSC Code:

12161700

NACRES:

NA.21

MDL number:

MFCD00131855

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Service technique

Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.

Laissez-nous vous aider

Propriétés

Nom du produit

Solution tampon phosphate, pH 7.4, pHast Pack^™, powder

product line

pHast Pack^™

form

powder

storage condition

(Tightly closed. Dry)

impurities

<5—2 ppm Heavy metals (Lead)

color

white

pH

7.4

solubility

soluble, clear, colorless

cation traces

Fe: <10 ppm

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

storage temp.

room temp

Description

General description

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Application

Phosphate buffered saline is commonly used in washing solutions for:

ELISA
Western Blotting and other protein applications
Cell culture (sterile filtration recommended)
Resuspension and sonication of cells
Immunohistochemistry

Features and Benefits

Save time with our ready-to-use formulation
No calculations, measurements, and adjusting pH
Reliable quality for reproducibility
Suitable for use in cell culture and electrophoresis
Biological tests: free of DNase, RNase, Protease, and Nickase
Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 1

Cet article	79378	P5119	P4474
Sigma-Aldrich PPB006 Solution tampon phosphate	Sigma-Aldrich 79378 Solution tampon phosphate	Sigma-Aldrich P5119 Solution tampon phosphate	Sigma-Aldrich P4474 Solution tampon phosphate
product line pHast Pack^™	product line BioUltra	product line -	product line -
form powder	form solution	form liquid	form liquid
solubility soluble, clear, colorless	solubility -	solubility -	solubility -
storage condition (Tightly closed. Dry)	storage condition -	storage condition -	storage condition -
storage temp. room temp	storage temp. -	storage temp. 2-8°C	storage temp. -
pH 7.4	pH 7.1-7.3	pH 7.2 (25 °C)	pH 7.4

Still not finding the right product?

Explore all of our products under Solution tampon phosphate

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Questions

1–3 of 3 Questions

10 months ago

What is the weight and dimensions of the packaging?

1 answer
1. Technical Support
  
  9 months ago
  
  The weight of the packaging is 160 grams.
  Regarding the dimensions, We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Documentation" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
  
  Helpful?
Anonymous

11 months ago

How can I determine the shelf life / expiration / retest date of this product?

1 answer
1. Technical Support
  
  11 months ago
  
  If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
  For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
  For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
  For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
  
  Helpful?
Anonymous

a year ago

How is shipping temperature determined? And how is it related to the product storage temperature?

1 answer
1. Technical Support
  
  a year ago
  
  Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
  
  Helpful?

Reviews

No rating value