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Key Documents

MAB1273C3

Sigma-Aldrich

Anti-Mitochondria Antibody, clone 113-1, Cy3 Conjugate

clone 113-1, from mouse, CY3 conjugate

Synonyme(s) :

Human Mitochondria

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

CY3 conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

113-1, monoclonal

Espèces réactives

human (mitochondria protein)

Ne doit pas réagir avec

rat (mitochondria protein), mouse (mitochondria protein)

Technique(s)

immunocytochemistry: suitable

Isotype

IgG1

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273C3 recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.

Spécificité

This antibody recognizes human. Antibody is not reactive with mouse or rodent.

Immunogène

Surface of intact human mitochondria

Application

Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1 & Cy3 Conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).
Research Category
Stem Cell Research

Cell Structure
Research Sub Category
Developmental Neuroscience

Organelle & Cell Markers

Qualité

Evaluated by Immunocytochemistry in neonatal human fibroblasts cells (SCC058).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in neonatal human fibroblast cells (SCC058).

Description de la cible

60 kDa calculated

Forme physique

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Stockage et stabilité

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Remarque sur l'analyse

Control
Neonatal human fibroblasts cells (SCC058).

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sarah Guégan et al.
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Jennifer A Kashatus et al.
Molecular cell, 57(3), 537-551 (2015-02-07)
Ras is mutated in up to 30% of cancers, including 90% of pancreatic ductal adenocarcinomas, causing it to be constitutively GTP-bound, and leading to activation of downstream effectors that promote a tumorigenic phenotype. As targeting Ras directly is difficult, there
Marta Aguado-Garrido et al.
International journal of molecular sciences, 25(3) (2024-02-10)
The treatment of spinal cord injury (SCI) with uncultivated human bone marrow-derived stromal cells (bmSCs) prepared by negative selection has been proposed to be therapeutically superior to treatment with stem cells that were expanded in vitro. To explore their use
Lorenzo Romero-Ramírez et al.
Journal of biomedical science, 27(1), 35-35 (2020-02-19)
Spinal cord injury (SCI) is a highly debilitating pathology without curative treatment. One of the most promising disease modifying strategies consists in the implantation of stem cells to reduce inflammation and promote neural regeneration. In the present study we tested
Roza K Przanowska et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 7687-7702 (2020-04-12)
miR-206, miR-1a-1, and miR-1a-2 are induced during differentiation of skeletal myoblasts and promote myogenesis in vitro. miR-206 is required for skeletal muscle regeneration in vivo. Although this miRNA family is hypothesized to play an essential role in differentiation, a triple

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