IP04
Protein G Plus-Agarose Suspension
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
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About This Item
Code UNSPSC :
41116133
Nomenclature NACRES :
NA.56
Produits recommandés
Forme
slurry (Liquid)
Contient
≤0.1% sodium azide as preservative
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Technique(s)
immunoprecipitation (IP): suitable
Adéquation
suitable for immunoprecipitation
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
Avertissement
Toxicity: Standard Handling (A)
Autres remarques
Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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