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PROTGA-RO

Roche

Protein G Agarose

>98% (HPLC and SDS-PAGE), suspension

Synonyme(s) :

agarose, protein g

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A propos de cet article

UNSPSC Code:
41116133
NACRES:
NA.54
Form:
suspension
Assay:
>98% (HPLC and SDS-PAGE)
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assay

>98% (HPLC and SDS-PAGE)

form

suspension

packaging

pkg of 15 mL (05015952001 [settled resin volume]), pkg of 2 mL (11719416001 [settled resin volume]), pkg of 5 mL (11243233001 [settled resin volume])

manufacturer/tradename

Roche

storage temp.

2-8°C

General description

Protein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30,000Da. It interacts with all subclasses of human IgG and also binds to mouse, rabbit and goat IgG.

Application

Affinity chromatography using Protein G Agarose, like Protein A Agarose, is the method of choice for purification of IgG from many species. It has been used as a pre-clearing agent during-
  • Ubiquitination assay
  • Immunoprecipitation

Biochem/physiol Actions

Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8)
Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel.

Features and Benefits

Contents
Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile.
Protein G content: 2.5mg/ml preswollen gel

Analysis Note

Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3

Classe de stockage

3 - Flammable liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

hcodes

H226,H319

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