IP05
Protein G Plus/Protein A Agarose Suspension
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Synonyme(s) :
Affinity resin, Protein G/Agarose
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About This Item
Code UNSPSC :
41116133
Nomenclature NACRES :
NA.56
Produits recommandés
Forme
slurry (Liquid)
Contient
≤0.1% sodium azide as preservative
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Technique(s)
protein purification: suitable
Adéquation
suitable for microbiology
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Application
Immunoprecipitation (see comments)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
33% slurry in PBS.
Autres remarques
Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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