S1629

Sulfatase from Aerobacter aerogenes

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

• Synonym(s): Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
• CAS Number: 9016-17-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.1.6.1 (BRENDA, IUBMB)
• MDL number: MFCD00132399
• Specific activity: 2-5 units/mg protein (biuret)
• Concentration: 10-20 units/mL

Properties

• type: Type VI
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• specific activity: 2-5 units/mg protein (biuret)
• mol wt: ~41 kDa
• concentration: 10-20 units/mL
• foreign activity: β-Glucuronidase ≤10 U/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.[1]

Application

Sulfatase from Aerobacter aerogenes has been used:

• as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses[2]
• in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1[1]
• to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides[3]

Biochem/physiol Actions

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging[4] and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis.[1] Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates.[5] Sulfatases find application in industry and agriculture.[1]

Physical form

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable