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RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217820

Pricing and availability is not currently available.

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Properties

Product Name

RPMI-1640 Medium, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO₃: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used

to obtain spleen and lymph nodes from mice[1]
for the resuspension of Staphylococcus aureus strains in the phagocytosis assay[2]
in the isolation of hepatitis B surface antigen-specific B-cell clones

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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This Item	R0883	R7509	R8758
Sigma-Aldrich R7638 RPMI-1640 Medium	Sigma-Aldrich R0883 RPMI-1640 Medium	Sigma-Aldrich R7509 RPMI-1640 Medium	Sigma-Aldrich R8758 RPMI-1640 Medium
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
components sodium pyruvate: no HEPES: 20 mM L-glutamine: no NaHCO₃: yes phenol red: yes	components sodium pyruvate: no phenol red: yes L-glutamine: no NaHCO₃: yes HEPES: no	components phenol red: no L-glutamine: no HEPES: no sodium pyruvate: no NaHCO₃: yes	components NaHCO₃: yes phenol red: yes sodium pyruvate: no HEPES: no L-glutamine: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
R7638-6X500ML	04061836696573
R7638-500ML	04061836696542

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